Healthcare

MTB-RESIST-I-TEST – TB First-Line Drug Resistance Detection Kit


MTB-RESIST-I-TEST first-line TB drug resistance detection kit

The kit is designed for detection of mutations associated with drug resistance in the tuberculosis complex mycobacteria DNA with determination of sensitivity to the First-line chemotherapy drugs and their analogues.
• The result of the assay allows for choosing of an appropriate therapy for patients with the confirmed diagnosis of pulmonary and extrapulmonary tuberculosis.
• The resistance of the tuberculosis complex mycobacteria to rifampicin (rpoB), isoniazid (katG and inhA) and their analogues is determined.
The kit is compatible with almost all RT-PCR devices.

Features & Characteristics

Detection Method Real-Time PCR (Melting Curves)
Test Format Qualitative Multiplex
Sample Sputum, bronchoalveolar lavage, bronchial washing, gastric washing, pleural Fluid, blood, urine, microbiological cultures, prostate secretion, tissue (biopsy and surgical) material, synovial Fluid, pericardial Fluid and cerebrospinal Fluid
Detection Of Mutations rpoB: Polymorphisms of codons 510–533, D516V, D516Y, codon 526, L533R, L533P, S531L
katG: S315T, S315N, S315R, S315I polymorphisms
inhA: C-15T polymorphism, and polymorphisms of the region -20 to +6 are also detected, but not differentiated
Number of Reactions 96 Reactions
Test Time 125~150 Minutes
Recommended Extraction Kit NA-Extra-M

Test Method

MTB-RESIST-I-TEST first-line Kit Test Method