TARF – Targeted Antitumor Response Panel

NGS-Based Mutation Analysis for Precision Oncology

TARF is a next-generation sequencing (NGS) assay designed to determine the mutation status of clinically significant gene regions associated with sensitivity to antitumor drug treatment. The panel supports precision oncology by enabling informed therapeutic decisions based on molecular profiling.

Genes Covered

• PIK3CA
• ESR1
• BRAF
• KRAS
• NRAS
• EGFR
• ERBB2

Clinical Applications

• Assessment of tumor sensitivity to targeted antitumor therapies
• Selection and optimization of personalized cancer treatment strategies
• Molecular profiling in solid tumor diagnostics
• Oncology research and translational medicine

Key Benefits

• Fast and cost-effective NGS workflow compared to conventional methods
• Significant reduction in library preparation time
• Indexing included within the target multiplex PCR stage
• High coverage uniformity and on-target specificity
• Automated bioinformatics analysis using XplainBio platform

Detected Target Regions (Selected)

• ESR1: Exons 1, 2, 4, 6, 7 (key codons including p75, p160, p327, p418–p514)
• PIK3CA: Exons 2, 5, 8, 21 (hotspot codons p38–p1049)
• BRAF, KRAS, NRAS, EGFR, ERBB2: Clinically relevant mutation regions

Technology Overview

• Analysis Type: Qualitative massive parallel sequencing (NGS)
• Target Enrichment: Multiplex PCR with integrated indexing
• Read Length: 2 × 150 bp
• Unique Molecular Identifiers (UMIs): Included for improved accuracy

Sample Compatibility

• Sample Types: Blood plasma, FFPE tissue blocks
• Recommended DNA Input: ≥ 10 ng

Technical Specifications

Parameter	Specification
Number of Reactions	96
Coverage Uniformity	99%
On-Target Rate	99%
Average Library Length	300 bp
Required Reads per Sample	100,000
Maximum Libraries per MiSeq Run	384
Library Preparation Time (96 samples)	~8.5 hours
Manual Work Time (96 samples)	~4 hours

Workflow Stages

1. Target multiplex PCR and index PCR
2. Library pooling and purification
3. NGS sequencing
4. Automated variant interpretation

Configuration Forms

• TARF-EP
• TARF-EBK
• TARF-P
• TARF-BNK
• TARF-7

Data Interpretation

TARF integrates with the XplainBio automated bioinformatics system, enabling simplified data interpretation, rapid variant reporting, and reduced analytical workload for laboratories.

Intended Use

• Clinical molecular diagnostic laboratories
• Oncology hospitals and cancer treatment centers
• Genetic testing and precision medicine facilities
• Research and academic institutions

Important Notes

• For professional in-vitro diagnostic use only
• Results must be interpreted by qualified specialists
• Genetic findings should be correlated with clinical data
• This assay supports therapy decisions but does not replace clinical judgment